Web2X Rapid Ligation Buffer. 1.5ml. Designed for use with T4 DNA Ligase to catalyze the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. Ligate DNA inserts into plasmid vectors in as little as 15 minutes. No purification necessary prior to transformation of ...

T4 DNA Ligase Ligase DNA Ligation - Promega

Web• 1 μL of T4 DNA ligase (Promega) • x μL water up to 20 μL total. *BsaI-HF is optimized for different buffer conditions and doesn't work well ** Promega T4 Ligase buffer has been shown to offer the highest activity for BsaI enzyme. Other T4 ligase enzymes will work, but Promega enzyme with Promega buffer is highest efficiency. WebThe enzyme most commonly used is derived from bacteriophage T4. T4 DNA Ligase is about 400-fold more active than E. coli DNA ligase for ligating blunt ends, and thus is the enzyme of choice for all molecular biology requirements. Promega offers T4 DNA Ligase in standard (Cat.# M1801) or high-concentrate (Cat.# middlesex county family court phone number

Tips for Maximizing Ligation Efficiencies NEB

WebT4 DNA Ligase Joins two dsDNA strands with cohesive or blunt ends by using the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. M1801, M1804, M1794 … WebT4 DNA Ligase, Blue/White Cloning Qualified Protocol. T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of … WebJul 8, 2024 · The PCR fragment is directly ligated to the T-tailed plasmid vector with no need for further enzymatic treatment other than the action of T4 DNA ligase. If desired, the insert can then be easily transferred from the T vector to other plasmids using the restriction sites present in the T vector. middlesex county fair nj