WebJun 17, 2013 · Evaluation of the expression of epithelial markers such as cytokeratin AE1, CAM5.2, EMA, and OSCAR is routinely used to identify poorly differentiated carcinoma. 4 Some early studies showed that some cytokeratins, such as AE1, are specific and helpful in distinguishing carcinoma from lymphoma and melanoma. 5 However, subsequent … WebThe cocktail of clones AE1 and AE3 exhibit broad reactivity with two families of cytokeratin, acidic and basic. Disclaimer Cytokeratin, Multi (AE1/AE3) is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains.
Cytokeratin - an overview ScienceDirect Topics
WebCytokeratins (AE1/AE3 [Fig. 25.55], CAM5.2) and epithelial membrane antigen (EMA) are the most commonly identified “epithelial” markers, present in up to 80% of cases, but … WebAug 8, 2024 · For cohort 2, 5 μm serial sections were stained with hematoxylin and eosin (H&E) or the following antibodies: S100 to highlight nerves, cytokeratin AE1/AE3 to highlight epithelial cells, and myelin basic protein (MBP) for validation of spatial transcriptomic data. Details are in Supplementary Materials and Methods. cif a08000234
Pathology Outlines - Cytokeratin AE1 / AE3
WebCytokeratin (AE1/AE3, CAM5.2) and epithelial membrane antigen are the most commonly identified “epithelial” markers, present in up to 80% of cases, but often limited to focal … WebThe immunohistochemistry reactions for the S100 protein, beta-catenin (Figure 3C), cytokeratin AE1/AE3, CD34 and progesterone receptor (PGR) were negative. The cell nuclei were negative for Ki-67, confirming their low proliferative potential. These results were typical of a benign tumor with smooth muscle differentiation; thus, the diagnosis of ... WebRegions within tumour cores were segmented using cytokeratin/ non-cytokeratin immunofluorescent masks to enable transcriptome and proteomic marker measurement within the tumour and stroma compartments independently (Figure S2). In this way, the measurements obtained from tumour regions comprised both tumour cells as well as … c++ if 2 conditions