Clarkson chromatography
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Clarkson chromatography
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WebClarkson Chromatography, USA). The following solvent regime was used to separate lipids according to polarity, with fraction 5 eluting the most polar lipids (Leblond and Chapman 2000): (1) 12 mL methylene chloride (hydrocarbons and sterol esters), (2) 15 ml 5 % acetone in methylene chloride with 0.05 % acetic acid (free t t
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